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modified langendorff apparatus hugo sachs elektronik  (Hugo Sachs Elektronik)

 
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    Hugo Sachs Elektronik modified langendorff apparatus hugo sachs elektronik
    Modified Langendorff Apparatus Hugo Sachs Elektronik, supplied by Hugo Sachs Elektronik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+langendorff+apparatus/langendorff+apparatus+hugo+sachs/pm31916218-50-7-9
    Average 90 stars, based on 1 article reviews
    modified langendorff apparatus hugo sachs elektronik - by Bioz Stars, 2026-10
    90/100 stars

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    Modification:

    Article Title: Role of mitochondrial permeability transition pore and mitochondrial ATP-sensitive potassium channels in the protective effects of ischemic preconditioning in isolated hearts from fed and fasted rats.
    Article Snippet: The aim of the present study was to assess whether the protective effects of ischemic preconditioning (PC) are associated with activation of the mitochondrial ATP-sensitive potassium channels (mitoKATP) and if there is any relationship between the activity of these channels and the mitochondrial permeability transition pore (MPTP) opening in ischemic-reperfused rat hearts under different nutritional conditions.. Langendorffperfused hearts of fed and 24-h fasted rats were exposed to 25 min of no-flow global ischemia plus 30 min of reperfusion.. Fasting accelerated functional recovery and attenuated MPTP opening.

    Article Title: Effects of the AMP-activated protein kinase inhibitor compound C on the postconditioned rat heart.
    Article Snippet: .. Hearts were then mounted on a modified Langendorff apparatus (Hugo Sachs Elektronik, March-Hugstetten, Germany) and perfused at a constant pressure of 70 mmHg with a non-recirculating Krebs–Ringer bicarbonate solution of the following composition (mM): NaCl 120, NaHCO3 25, KCl 4.8, MgSO4 1.33, KH2PO4 1.2, CaCl2 1.6, Na2EDTA 0.02, glucose 10. ..

    Article Title: Effects of the AMP-activated protein kinase inhibitor compound C on the postconditioned rat heart
    Article Snippet: .. Hearts were then mounted on a modified Langendorff apparatus (Hugo Sachs Elektronik, March-Hugstetten, Germany) and perfused at a constant pressure of 70 mmHg with a non-recirculating Krebs–Ringer bicarbonate solution of the following composition (mM): NaCl 120, NaHCO 3 25, KCl 4.8, MgSO 4 1.33, KH 2 PO 4 1.2, CaCl 2 1.6, Na 2 EDTA 0.02, glucose 10. ..

    Article Title: Involvement of energetic metabolism in the effects of ischemic postconditioning on the ischemic-reperfused heart of fed and fasted rats
    Article Snippet: .. Hearts were then mounted on a modified Langendorff apparatus (Hugo Sachs Elektronik, March-Hugstetten, Germany) and perfused at a constant pressure of 70 mmHg with a non-recirculating Krebs-Ringer bicarbonate solution of the following composition (mM): NaCl 120; NaHCO 3 25; KCl 4.8; MgSO 4 1.33; KH 2 PO 4 1.2; CaCl 2 1.6; Na 2 EDTA 0.02; glucose 10. ..

    Article Title: Restricted feeding improves postischemic recovery of Langendorff-perfused rat hearts.
    Article Snippet: The goal of the present study was to assess the effects of a restricted feeding schedule (RFS) on postischemic contractile recovery in relation to triacylglycerol (TAG), glycogen, and ATP content.. Glucose-6-phosphate dehydrogenase (G6PDH) activity, reduced/oxidized glutathione ratio (GSH/GSSG), and thiobarbituric acid reactive substances (TBARS) levels were also determined.. Isolated rat hearts entrained to daily RFS (2 h food access starting at 1200) or fed ad libitum (FED) for 3 weeks were Langendorff-perfused (25 min ischemia, 30 min reperfusion) with Krebs–Ringer bicarbonate solution (10 mmol/L glucose).

    Article Title: Involvement of mitochondrial permeability transition, glutathione status, pentose phosphate pathway and oxidative damage in the protective effect of fasting on the ischaemic-reperfused rat heart.
    Article Snippet: Fasting, which increases the catabolism of fatty acids, gives functional protection to the ischaemic–reperfused heart.. To obtain further knowledge of this cardioprotective effect, changes in mitochondrial permeability transition (MPT) were measured by the entrapment of 2-deoxy-[H]-glucose (2-DG).. We also assessed whether MPT is associated with changes in glutathione status, the activity of glucose-6-phosphate-dehydrogenase (G6PDH) and tissue oxidative damage, estimated by the measurement of Thiobarbituric acid-reactive substances (TBARS).

    Article Title: Involvement of energetic metabolism in the effects of ischemic postconditioning on the ischemic-reperfused heart of fed and fasted rats.
    Article Snippet: .. Hearts were then mounted on a modified Langendorff apparatus (Hugo Sachs Elektronik, March-Hugstetten, Germany) and perfused at a constant pressure of 70 mmHg with a non-recirculating Krebs-Ringer bicarbonate solution of the following composition (mM): NaCl 120; NaHCO3 25; KCl 4.8; MgSO4 1.33; KH2PO4 1.2; CaCl2 1.6; Na2EDTA 0.02; glucose 10. ..



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    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, <t>Langendorff-perfused</t> hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.
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    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, <t>Langendorff-perfused</t> hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.
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    Image Search Results


    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, Langendorff-perfused hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.

    Journal: Nature communications

    Article Title: The mitochondrial uniporter controls fight or flight heart rate increases

    doi: 10.1038/ncomms7081

    Figure Lengend Snippet: a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, Langendorff-perfused hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.

    Article Snippet: Briefly, excised hearts from 7–9 week female and male mice were rapidly mounted on a modified Langendorff apparatus (HSE-HA perfusion systems, Harvard Apparatus, Holliston, Mass) for retrograde aortic perfusion at a constant pressure of 80 mm Hg with oxygenated (95% O 2 , 5% CO 2 ) Krebs-Henseleit solution consisting of (mM) 25.0 NaHCO 3 , 118.5 NaCl, 4.0 KCl, 1.2 MgSO 4 , 1.2 NaH 2 PO 4 , 1.5 CaCl 2 , and 11.2 glucose, with pH equilibrated to 7.4.

    Techniques: Staining, Expressing, Ex Vivo, Isolation